Holographic Coulomb Branch Flows with N=1 Supersymmetry

We obtain a large, new class of N=1 supersymmetric holographic flow backgrounds with U(1)^3 symmetry. These solutions correspond to flows toward the Coulomb branch of the non-trivial N=1 supersymmetric fixed point. The massless (complex) chiral fields are allowed to develop vevs that are independent of their two phase angles, and this corresponds to allowing the brane to spread with arbitrary, U(1)^2 invariant, radial distributions in each of these directions. Our solutions are "almost Calabi-Yau:" The metric is hermitian with respect to an integrable complex structure, but is not Kahler. The "modulus squared" of the holomorphic (3,0)-form is the volume form, and the complete solution is characterized by a function that must satisfy a single partial differential equation that is closely related to the Calabi-Yau condition. The deformation from a standard Calabi-Yau background is driven by a non-trivial, non-normalizable 3-form flux dual to a fermion mass that reduces the supersymmetry to N=1. This flux also induces dielectric polarization of the D3-branes into D5-branes.Comment: 22 pages; harvmac. Typos corrected;small improvements in presentatio

Can tillage and agronomy be integrated with herbicide application to control resistant weeds?

Non-Peer ReviewedThe prevalence of group 2 resistant broadleaved weeds threatens successful lentil production on the Canadian Great Plains. The objective of this study was to develop an integrated weed management strategy combining physical, cultural and chemical weed control methods for lentil producers dealing with group 2 resistant wild mustard. The study was conducted for 3 years between 2011 and 2013 at 2 locations at Saskatoon and Scott, Saskatchewan. It was a randomized two way factorial with weed control method and seeding rate as the main effects. Weed control treatments tested consisted of a control treated with a glyphosate burnoff, saflufenacil (Heat ™) herbicide, rotary hoeing, half rate metribuzin (Sencor ™) herbicide, a fully integrated treatment, and a full herbicide treatment. Three seeding rates representing 1, 2, and 4 times the recommended seeding rate were tested (130, 260, and 520 plants m-2). Increasing seeding rate consistently lowered mustard biomass at both locations. The full herbicide treatment provided the greatest reduction in mustard biomass followed by the integrated treatment. The integrated treatment relied more on increased seeding rate to reduce mustard biomass and produce yield, and at the highest seeding rate it was able to provide equivalent yield to the full herbicide system. The results of this study show that an integrated system utilizing an increased seeding rate can control resistant weeds and maintain yields to a similar level as a strategy that relies only on herbicides for weed control

Flowing with Eight Supersymmetries in M-Theory and F-theory

We consider holographic RG flow solutions with eight supersymmetries and study the geometry transverse to the brane. For both M2-branes and for D3-branes in F-theory this leads to an eight-manifold with only a four-form flux. In both settings there is a natural four-dimensional hyper-Kahler slice that appears on the Coulomb branch. In the IIB theory this hyper-Kahler manifold encodes the Seiberg-Witten coupling over the Coulomb branch of a U(1) probe theory. We focus primarily upon a new flow solution in M-theory. This solution is first obtained using gauged supergravity and then lifted to eleven dimensions. In this new solution, the brane probes have an Eguchi-Hanson moduli space with the M2-branes spread over the non-trivial 2-sphere. It is also shown that the new solution is valid for a class of orbifold theories. We discuss how the hyper-Kahler structure on the slice extends to some form of G-structure in the eight-manifold, and describe how this can be computed.Comment: 29 pages, 1 figure, harvma

Single-molecule study of redox control involved in establishing the spinach plastocyanin-cytochrome b6f electron transfer complex

Small diffusible redox proteins play a ubiquitous role in bioenergetic systems, facilitating electron transfer (ET) between membrane bound complexes. Sustaining high ET turnover rates requires that the association between extrinsic and membrane-bound partners is highly specific, yet also sufficiently weak to promote rapid post-ET separation. In oxygenic photosynthesis the small soluble electron carrier protein plastocyanin (Pc) shuttles electrons between the membrane integral cytochrome b6f (cytb6f) and photosystem I (PSI) complexes. Here we use peak-force quantitative nanomechanical mapping (PF-QNM) atomic force microscopy (AFM) to quantify the dynamic forces involved in transient interactions between cognate ET partners. An AFM probe functionalised with Pc molecules is brought into contact with cytb6f complexes, immobilised on a planar silicon surface. PF-QNM interrogates the unbinding force of the cytb6f-Pc interactions at the single molecule level with picoNewton force resolution and on a time scale comparable to the ET time in vivo (ca. 120 μs). Using this approach, we show that although the unbinding force remains unchanged the interaction frequency increases over five-fold when Pc and cytb6f are in opposite redox states, so complementary charges on the cytb6f and Pc cofactors likely contribute to the electrostatic forces that initiate formation of the ET complex. These results suggest that formation of the docking interface is under redox state control, which lowers the probability of unproductive encounters between Pc and cytb6f molecules in the same redox state, ensuring the efficiency and directionality of this central reaction in the ‘Z-scheme’ of photosynthetic ET

The complex geometry of holographic flows of quiver gauge theories

We argue that the complete Klebanov-Witten flow solution must be described by a Calabi-Yau metric on the conifold, interpolating between the orbifold at infinity and the cone over T^(1,1) in the interior. We show that the complete flow solution is characterized completely by a single, simple, quasi-linear, second order PDE, or "master equation," in two variables. We show that the Pilch-Warner flow solution is almost Calabi-Yau: It has a complex structure, a hermitian metric, and a holomorphic (3,0)-form that is a square root of the volume form. It is, however, not Kahler. We discuss the relationship between the master equation derived here for Calabi-Yau geometries and such equations encountered elsewhere and that govern supersymmetric backgrounds with multiple, independent fluxes.Comment: 26 pages, harvmac + amssy

Interference lithographic nanopatterning of plant and bacterial light-harvesting complexes on gold substrates

We describe a facile approach for nanopatterning of photosynthetic light-harvesting complexes over macroscopic areas, and use optical spectroscopy to demonstrate retention of native properties by both site-specifically and non-specifically attached photosynthetic membrane proteins. A Lloyd's mirror dual-beam interferometer was used to expose self-assembled monolayers of amine-terminated alkylthiolates on gold to laser irradiation. Following exposure, photo-oxidized adsorbates were replaced by oligo(ethylene glycol) terminated thiols, and the remaining intact amine-functionalized regions were used for attachment of the major light-harvesting chlorophyll–protein complex from plants, LHCII. These amine patterns could be derivatized with nitrilotriacetic acid (NTA), so that polyhistidine-tagged bacteriochlorophyll–protein complexes from phototrophic bacteria could be attached with a defined surface orientation. By varying parameters such as the angle between the interfering beams and the laser irradiation dose, it was possible to vary the period and widths of NTA and amine-functionalized lines on the surfaces; periods varied from 1200 to 240 nm and linewidths as small as 60 nm (λ/4) were achieved. This level of control over the surface chemistry was reflected in the surface topology of the protein nanostructures imaged by atomic force microscopy; fluorescence imaging and spectral measurements demonstrated that the surface-attached proteins had retained their native functionality

Factors affecting success of conservation translocations of terrestrial vertebrates: a global systematic review

Translocation—moving individuals for release in different locations—is among the most important conservation interventions for increasing or re-establishing populations of threatened species. However, translocations often fail. To improve their effectiveness, we need to understand the features that distinguish successful from failed translocations. We assembled and analysed a global database of translocations of terrestrial vertebrates (n = 514) to assess the effects of various design features and extrinsic factors on success. We analysed outcomes using standardised metrics: a categorical success/failure classification; and population growth rate. Probability of categorical success and population growth rate increased with the total number of individuals released but with diminishing returns above about 20–50 individuals. Positive outcomes—categorical success and high population growth—were less likely for translocations in Oceania, possibly because invasive species are a major threat in this region and are difficult to control at translocation sites. Rates of categorical success and population growth were higher in Europe and North America than elsewhere, suggesting the key role of context in positive translocation outcomes. Categorical success has increased throughout the 20th century, but that increase may have plateaued at about 75% since about 1990. Our results suggest there is potential for further increase in the success of conservation translocations. This could be best achieved by greater investment in individual projects, as indicated by total number of animals released, which has not increased over time.Shane D. Morris, Barry W. Brook, Katherine E. Moseby, Christopher N. Johnso

Nanodomains of Cytochrome b(6)f and Photosystem II Complexes in Spinach Grana Thylakoid Membranes

The cytochrome b6f (cytb6f) complex plays a central role in photosynthesis, coupling electron transport between photosystem II (PSII) and photosystem I to the generation of a transmembrane proton gradient used for the biosynthesis of ATP. Photosynthesis relies on rapid shuttling of electrons by plastoquinone (PQ) molecules between PSII and cytb6f complexes in the lipid phase of the thylakoid membrane. Thus, the relative membrane location of these complexes is crucial, yet remains unknown. Here, we exploit the selective binding of the electron transfer protein plastocyanin (Pc) to the lumenal membrane surface of the cytb6f complex using a Pc-functionalized atomic force microscope (AFM) probe to identify the position of cytb6f complexes in grana thylakoid membranes from spinach (Spinacia oleracea). This affinity-mapping AFM method directly correlates membrane surface topography with Pc-cytb6f interactions, allowing us to construct a map of the grana thylakoid membrane that reveals nanodomains of colocalized PSII and cytb6f complexes. We suggest that the close proximity between PSII and cytb6f complexes integrates solar energy conversion and electron transfer by fostering short-range diffusion of PQ in the protein-crowded thylakoid membrane, thereby optimizing photosynthetic efficiency

Correlated fluorescence quenching and topographic mapping of Light-Harvesting Complex II within surface-assembled aggregates and lipid bilayers

Light-Harvesting Complex II (LHCII) is a chlorophyll-protein antenna complex that efficiently absorbs solar energy and transfers electronic excited states to photosystems I and II. Under excess light intensity LHCII can adopt a photoprotective state in which excitation energy is safely dissipated as heat, a process known as Non-Photochemical Quenching (NPQ). In vivo NPQ is triggered by combinatorial factors including transmembrane ΔpH, PsbS protein and LHCII-bound zeaxanthin, leading to dramatically shortened LHCII fluorescence lifetimes. In vitro, LHCII in detergent solution or in proteoliposomes can reversibly adopt an NPQ-like state, via manipulation of detergent/protein ratio, lipid/protein ratio, pH or pressure. Previous spectroscopic investigations revealed changes in exciton dynamics and protein conformation that accompany quenching, however, LHCII-LHCII interactions have not been extensively studied. Here, we correlated fluorescence lifetime imaging microscopy (FLIM) and atomic force microscopy (AFM) of trimeric LHCII adsorbed to mica substrates and manipulated the environment to cause varying degrees of quenching. AFM showed that LHCII self-assembled onto mica forming 2D-aggregates (25–150 nm width). FLIM determined that LHCII in these aggregates were in a quenched state, with much lower fluorescence lifetimes (~0.25 ns) compared to free LHCII in solution (2.2–3.9 ns). LHCII-LHCII interactions were disrupted by thylakoid lipids or phospholipids, leading to intermediate fluorescent lifetimes (0.6–0.9 ns). To our knowledge, this is the first in vitro correlation of nanoscale membrane imaging with LHCII quenching. Our findings suggest that lipids could play a key role in modulating the extent of LHCII-LHCII interactions within the thylakoid membrane and so the propensity for NPQ activation

Intersecting M-branes and bound states

In this paper, we construct multi-scalar, multi-center $p$-brane solutions in toroidally compactified M-theory. We use these solutions to show that all supersymmetric $p$-branes can be viewed as bound states of certain basic building blocks, namely $p$-branes that preserve $1/2$ of the supersymmetry. We also explore the M-theory interpretation of $p$-branes in lower dimensions. We show that all the supersymmetric $p$-branes can be viewed as intersections of M-branes or boosted M-branes in $D=11$.Comment: Latex, 14 pages, no figures. References adde